Growth phase and ompR regulation of transcription of microcin B17 genes
نویسندگان
چکیده
منابع مشابه
Fragments of the Bacterial Toxin Microcin B17 as Gyrase Poisons
Fluoroquinolones are very important drugs in the clinical antibacterial arsenal; their success is principally due to their mode of action: the stabilisation of a gyrase-DNA intermediate (the cleavage complex), which triggers a chain of events leading to cell death. Microcin B17 (MccB17) is a modified peptide bacterial toxin that acts by a similar mode of action, but is unfortunately unsuitable ...
متن کاملa tripartite model of efl teachers attributions, burnout, and self-regulation: towards the prospects of effective teaching
همطالعه حاضر به ارائه مدلی برای آموزش موثر زبان انگلیسی می پردازد. مدل حاضر از سه عامل تاثیر گذار در کارایی تدریس معلمان زبان انگلیسی بهره می برد. این سه عامل شامل سبکهای اسنادی، خود تنطیمی و فرسودگی شغلی معلمان ایرانی زبان انگلیسی می باشد. رساله مورد نظر درچهار فاز طراحی شده است: فاز اول شامل طراحی و رواسازی پرسشنامه سبکهای اسنادی معلمان زبان انگلیسی و فاز دوم شامل استفاده از این پرسشنا...
Growth-phase-dependent expression of the cyclopeptide antibiotic microcin J25.
Microcin J25 is a 2,107-Da, plasmid-encoded, cyclopeptide antibiotic produced by Escherichia coli. We have isolated lacZ fusions to mcjA (encoding the 58-amino-acid microcin precursor) and mcjB and mcjC (which are required for microcin maturation), and the regulation of these fusions was used to identify factors that control the expression of these genes. The mcjA gene was found to be dramatica...
متن کاملCloning and mapping of the genetic determinants for microcin B17 production and immunity.
Plasmid pMccB17 (70 kilobases [kb]) codes for the production of microcin B17, a peptide that inhibits DNA synthesis, and for microcin B17 immunity. A BamHI-EcoRI fragment of 5.1 kb from pMccB17 was cloned into pBR322 in two steps. The resulting plasmid (pMM102) overproduced microcin B17 and expressed immunity against microcin. Mcc- and Mcc- Imm- mutants were isolated on plasmids pMccB17 and pMM...
متن کاملPosttranslational heterocyclization of cysteine and serine residues in the antibiotic microcin B17: distributivity and directionality.
To produce the antibiotic Microcin B17, four Cys and four Ser residues are converted into four thiazoles and four oxazoles by the three subunit Microcin B17 synthetase. High-resolution mass spectrometry (MS) was used to monitor the kinetics of posttranslational heterocyclic ring formation (-20 Da per ring) and demonstrated the accumulation of all intermediates, from one to seven rings, indicati...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1986
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.167.3.1058-1065.1986